_________ are established with cells that have been frozen in liquid nitrogen, thawed and then placed in a waiting culture medium.
A)Primary cultures
B)Secondary cultures
C)Tertiary cultures
D)Supernatant cultures
E)Penultimate cultures

Question

Quizplus · Accepted Answer

Secondary cultures are established with cells that have been frozen and thawed before being placed in a waiting culture medium. Primary cultures are derived directly from tissue, while tertiary cultures and penultimate cultures do not typically exist as defined terms in cell culture. Supernatant cultures refer to the liquid portion of a cell culture following centrifugation, rather than the cells themselves.

_________ Are Established with Cells That Have Been Frozen in Liquid