You isolate DNA from a particular organism and analyze it. The amount of adenine was 6 µmoles and the A+T/G+C ratio is 4.0. How much guanine should be in the sample?

Question

Quizplus · Accepted Answer

Given the A+T/G+C ratio is 4.0 and the amount of adenine (A) is 6 µmoles, the total amount of adenine and thymine (A+T) is 4 times the amount of guanine and cytosine (G+C). Since A = T in DNA, A+T = 12 µmoles. Therefore, G+C must be 3 µmoles (since 12 µmoles is 4 times 3 µmoles). Given that G = C in DNA, the amount of guanine (G) must be half of 3 µmoles, which is 1.5 µmoles.

You Isolate DNA from a Particular Organism and Analyze It