A control cell that is synthesizing a GFP-labeled version of mannosidase II has fluorescence localized in the Golgi complexes of the cell. Normally, this enzyme is synthesized in the endoplasmic reticulum and moves via transport vesicles to the Golgi complex, where it takes up residence. If an experimental cell contains an siRNA that leads to the fluorescence being restricted to the endoplasmic reticulum, with what would the siRNA be likely to interfere?
A)an mRNA that codes for a protein involved in the transport of the enzyme from the ER to the Golgi complex
B)an rRNA that synthesizes the enzyme
C)the synthesis of mannosidase II from its mRNA
D)an mRNA that codes for a protein involved in the transport of the enzyme from the Golgi complex to the ER
E)an mRNA that codes for the enzyme

Question

Quizplus · Accepted Answer

The siRNA is likely to interfere with the mRNA that codes for a protein involved in the transport of the enzyme from the ER to the Golgi complex, as this step is necessary for the normal targeting and localization of mannosidase II to the Golgi complex.

A Control Cell That Is Synthesizing a GFP-Labeled Version of Mannosidase