In the experiment that demonstrated that rotational catalysis occurs, a fluorescently labeled actin filament attached to the ATP synthase enzyme's ɤ subunit was seen to rotate when ATP was added to the enzyme, which was fixed to a cover slip. With each step in the rotational catalysis, how much could the actin filament be seen to rotate?

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Quizplus · Accepted Answer

The actin filament could be seen to rotate 120° with each step in the rotational catalysis. This is because ATP synthase operates through a mechanism where its ɤ subunit rotates within the enzyme, causing conformational changes that are necessary for ATP synthesis. The enzyme has three active sites that go through a cycle of ATP synthesis, and the 120° rotation corresponds to the transition between these states.

In the Experiment That Demonstrated That Rotational Catalysis Occurs, a Fluorescently