Green fluorescent protein (GFP) can be used to fluorescently label a specific protein in cells by genetically engineering cells to synthesize the target protein fused to GFP. What is the advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labeled probes that bind to the target protein?
A) GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires preserved cells.
B) GFP fusions enable higher resolution than staining with fluorescent probes.
C) GFP permits the position of the protein in the cell more precisely than fluorescent probes.
D) GFP permits visualization of protein-protein interactions; fluorescent probes do not.
E) GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.
Correct Answer:
Verified
Q12: The volume enclosed by the plasma membrane
Q13: The advantage of light microscopy over electron
Q14: The evolution of eukaryotic cells most likely
Q15: If radioactive deoxythymidine triphosphate (dTTP)is added to
Q16: Which organelle or structure is absent in
Q18: All of the following are part of
Q19: In the fractionation of homogenized cells using
Q20: Large numbers of ribosomes are present in
Q21: A cell with a predominance of free
Q22: The fact that the outer membrane of
Unlock this Answer For Free Now!
View this answer and more for free by performing one of the following actions
Scan the QR code to install the App and get 2 free unlocks
Unlock quizzes for free by uploading documents