Suppose the interior of the thylakoids of isolated chloroplasts were made acidic and then transferred in the dark to a pH 8 solution. What would be likely to happen?
A) The isolated chloroplasts will make ATP.
B) The Calvin cycle will be activated.
C) Cyclic photophosphorylation will occur.
D) The isolated chloroplasts will generate oxygen gas.
E) The isolated chloroplasts will reduce NADP⁺ to NADPH.

Question

Quizplus · Accepted Answer

The acidification of the thylakoid interior, followed by placement in a basic solution, creates a proton gradient across the thylakoid membrane. This gradient drives ATP synthesis through chemiosmosis, similar to the process occurring during the light reactions of photosynthesis, but without the need for light in this experimental setup.

Suppose the Interior of the Thylakoids of Isolated Chloroplasts Were