Use the following information to answer the questions below.
Nucleotides can be radiolabeled before they are incorporated into newly forming DNA and can therefore be assayed to track their incorporation. In a set of experiments, a student-faculty research team used labeled T nucleotides and introduced these into the culture of dividing human cells at specific times.
-Once they had determined which cells were dividing, the team wanted to use a non-radioactive method to track whether various physiological factors (such as food or body temperature)affect the action of the pathogen. Which of the following would be effective, simple, and safe?
A) measuring picograms of DNA
B) measuring numbers of chromosomes
C) measuring numbers of chromatids
D) counting the frequency of cells in mitosis
E) counting newly formed plasma membranes

Question

Quizplus · Accepted Answer

Counting the frequency of cells in mitosis would be the most effective and simple method to track the effect of physiological factors on DNA incorporation without using radioactive labeling. This method utilizes microscopy to identify dividing cells, and it is safe since it only involves observing the cells and does not require any chemical or biological manipulations. Measuring picograms of DNA or counting newly formed plasma membranes would not provide direct information on DNA incorporation and are more complicated methods. Measuring the number of chromosomes or chromatids may not accurately reflect DNA incorporation since abnormalities in chromosome structure or number can occur during cell division.

Use the Following Information to Answer the Questions Below