Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of
A) post-transcriptional processing.
B) electroporation.
C) post-translational processing.
D) nucleic acid hybridization.
E) restriction fragment ligation.

Question

Quizplus · Accepted Answer

Cloning a eukaryotic gene in a bacterial cell involves the challenge of post-transcriptional processing, which is different in eukaryotes and prokaryotes. Therefore, the use of mRNA and reverse transcriptase can help bypass this challenge by allowing for transcription of the gene in the bacterial cell.

Expression of a Cloned Eukaryotic Gene in a Bacterial Cell