The biggest problem with the shotgun technique is its tendency to underestimate the size of the genome. Which of the following might best account for this?
A) skipping some of the clones to be sequenced
B) missing some of the overlapping regions of the clones
C) counting some of the overlapping regions of the clones twice
D) having some of the clones die during the experiment and therefore not be represented
E) missing some duplicated sequences

Question

Quizplus · Accepted Answer

The shotgun technique can miss detecting duplicated sequences, which are common in many genomes. This can lead to an underestimation of genome size.

The Biggest Problem with the Shotgun Technique Is Its Tendency