A "normal", not deionized water was used in the experiment that included an enzymatic reaction. The experiment did not work as expected. What is the MOST LIKELY reason for the failure?
A) The "normal" water used in the experiment contained ions that interacted with the enzyme activity.
B) Deionized water is usually warmer, and the experimenter forgot to heat up the water from the "normal" faucet.
C) Deionized water most likely gave the false positive result due to lack of ions.
D) "Normal" water gave false negative results due to lack of necessary ions that deionized water has.

Question

Quizplus · Accepted Answer

Enzymatic reactions are highly sensitive to the presence of ions in the reaction mixture. The use of "normal" water, which is not deionized, may contain ions that can interact with the enzyme and disrupt the reaction. Therefore, option A is the most likely reason for the experiment's failure.

A "Normal", Not Deionized Water Was Used in the Experiment