To obtain the most accurate measure of resting metabolic rate in healthy adults, before beginning an indirect calorimetry procedure, the subject should abstain from moderate aerobic or anaerobic exercise for at least 2 hours

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This is true because engaging in moderate exercise prior to the measurement can elevate metabolic rate and result in an inaccurate reading. It is also recommended to avoid consuming caffeine or nicotine and to fast for several hours prior to the measurement for the most accurate result.

To Obtain the Most Accurate Measure of Resting Metabolic Rate