The restriction endonuclease EcoRI recognizes the sequence GAATTC and cuts it between G and A on both strands. Which of the following sticky ends will be produced when EcoRI cuts the template and complementary strands of the given nucleotide sequence?

Question

Quizplus · Accepted Answer

When EcoRI cuts the sequence GAATTC, the sticky ends produced will have a 5' overhang of AATT. Therefore, the correct sticky ends for the given nucleotide sequence will be:
Template strand: 5'- G A A T T C -3' (cut between G and A)
3'-overhang: AATT
Complementary strand: 3'- C T T A A G -5' (cut between C and T)
5'-overhang: TTAA
Option B is the only one that matches this criteria.

The Restriction Endonuclease EcoRI Recognizes the Sequence GAATTC and Cuts