Which of the Following Would Render an Autoclaved Load Unsterile $\degree$

Which of the following would render an autoclaved load unsterile?

A) Steam temperature of 212 $\degree$ F or 100 $\degree$ C
B) Wrapping the instruments in muslin
C) Air trapped inside the chamber because of blocked discharge lines
D) Placing hinged instruments in the open position
E) Refilling the water reservoir before operating