The sequence of a heptapeptide can be deduced from the following experimental results as Thr-Tyr-Cys-Gln-Arg-Trp-His
Edman degradation Thr
Hydrolysis catalyzed by Trypsin Thr-Tyr-Cys-Gln-Arg
Trp-His
Hydrolysis catalyzed by Chymotrypsin Thr-Tyr
Cys-Gln-Arg-Trp
His

Question

Quizplus · Accepted Answer

The Edman degradation indicates the N-terminal is Thr. Trypsin cleaves after Arg, and Chymotrypsin cleaves after Tyr, Trp, and Phe. The given results do not match the expected cleavage patterns for the sequence Thr-Tyr-Cys-Gln-Arg-Trp-His.

The Sequence of a Heptapeptide Can Be Deduced from the Following