The sequence of a decapeptide can be deduced from the following experimental results as Leu-Met-Ser-Thr-Trp-His-Phe-Asp-Lys-Glu.
Edman degradation Leu
Hydrolysis catalyzed by Trypsin Leu-Met-Ser-Thr-Trp-His-Phe-Asp-Lys
Glu
Hydrolysis catalyzed by Chymotrypsin Leu-Met-Ser-Thr-Trp
His-Phe
Asp-Lys-Glu
Treatment with Cyanogen Bromide Leu-Met
Ser-Thr-Trp-His-Phe-Asp-Lys-Glu

Question

Quizplus · Accepted Answer

The experimental results do not match the given sequence. Edman degradation identifies the N-terminal amino acid as Leu, which is consistent. Trypsin cleaves after Lys or Arg, suggesting a sequence ending with Lys. Chymotrypsin cleaves after aromatic amino acids (Phe, Trp, Tyr), indicating breaks after Trp and Phe. Cyanogen Bromide cleaves after Met, suggesting a break after Met. The given sequence does not align with these cleavage patterns.

The Sequence of a Decapeptide Can Be Deduced from the Following