Which two components of the Gram stain form a complex that is retained by Gram-positive cells?
A) crystal violet and iodine
B) safranin and iodine
C) crystal violet and safranin
D) alcohol and safranin
E) alcohol and iodine

Question

Quizplus · Accepted Answer

Crystal violet and iodine form a complex that is retained by the thick peptidoglycan layer of Gram-positive cells, leading to their purple color after staining. Gram-negative cells, on the other hand, have a thinner peptidoglycan layer that is unable to retain this complex and are decolorized by the alcohol wash.

Which Two Components of the Gram Stain Form a Complex