Which of these techniques can be used to localize the DNA sequence at the origin of replication in a bacterial cell?
A) fluorescence microscopy
B) phase contrast
C) X-ray diffraction
D) atomic force microscopy
E) cryo-EM

Question

Quizplus · Accepted Answer

Fluorescence microscopy can be used to locate specific DNA sequences using fluorescently labeled probes. In the case of bacterial replication, there are specific proteins that bind to the origin of replication, which can also be labeled with fluorescent probes. By using fluorescence microscopy, the location of the labeled probes can be visualized and the origin of replication identified. The other techniques listed are not typically used for DNA sequence localization in bacteria.

Which of These Techniques Can Be Used to Localize the DNA