In early cloning techniques, E. coli was ________ with the DNA of interest contained in a plasmid or phage vector. This cloned DNA could then be transferred to another organism using a ________.
A) conjugated; contig
B) conjugated; vector
C) transformed; vector
D) transformed; contig
E) transformed; polymerase chain reaction

Question

Quizplus · Accepted Answer

E. coli was transformed with the DNA of interest contained in a plasmid or phage vector, and this cloned DNA could then be transferred to another organism using a vector. Conjugation involves the transfer of genetic material between bacteria through direct contact, while contigs refer to overlapping DNA fragments that are used to reconstruct a complete genome sequence. Polymerase chain reaction (PCR) is a technique used to amplify DNA fragments, but it is not used to transfer cloned DNA to another organism.

In Early Cloning Techniques, E