Which of the following experimental procedures is NOT used in ChIP-seq?
A) DNA-protein complexes are cross-linked.
B) DNA is extracted and sheared.
C) Bead-bound antibodies to transcription factors are used to precipitate transcription factors cross-linked with DNA.
D) The bead-bound antibodies are precipitated using a secondary antibody.
E) Transcription factors are released from DNA, amplified and identified by sequencing.
Correct Answer:
Verified
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