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The Figure Shown Below Is a Picture of a Gel

Question 22

Multiple Choice

The figure shown below is a picture of a gel with multiplex PCR products from Clostridium botulinum isolates. Lane 1 contains DNA molecular weight markers and lanes 2-5 are positive strains as controls for toxins type A, type B, type E, and type F, respectively. Based on the bands shown, what can we say about the isolate results in lane 6? The figure shown below is a picture of a gel with multiplex PCR products from Clostridium botulinum isolates. Lane 1 contains DNA molecular weight markers and lanes 2-5 are positive strains as controls for toxins type A, type B, type E, and type F, respectively. Based on the bands shown, what can we say about the isolate results in lane 6?   A)  The isolate in lane 6 produces all four toxins but not endotoxin. B)  The PCR products from isolate in lane 6 are consistent with that isolate having toxins A, F, and B but not E. C)  The isolate in lane 6 produces all four toxins. D)  There is no band for endotoxin in lane 6 but the isolate makes three other toxins. E)  The gel shows definitive proof that this strain produces three toxins.


A) The isolate in lane 6 produces all four toxins but not endotoxin.
B) The PCR products from isolate in lane 6 are consistent with that isolate having toxins A, F, and B but not E.
C) The isolate in lane 6 produces all four toxins.
D) There is no band for endotoxin in lane 6 but the isolate makes three other toxins.
E) The gel shows definitive proof that this strain produces three toxins.

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