What clues provided evidence of the mechanism of channel inactivation?

Question

Quizplus · Accepted Answer

The evidence for the mechanism of channel inactivation includes trypsin digestion experiments showing that the channel remains open when the cytoplasmic side is digested (A), observations that protein mutants exhibit different inactivation kinetics (B), and the finding that inactivation can be restored by adding back a portion of a missing peptide (C). Together, these clues support the understanding of how channel inactivation occurs at a molecular level.

What Clues Provided Evidence of the Mechanism of Channel Inactivation