The specificity or stringency of a PCR reaction can be controlled by altering the reaction
A) volume.
B) target sequence.
C) temperature and salt concentration.
D) time.
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

The specificity or stringency of a PCR reaction is primarily controlled by adjusting the annealing temperature and the salt concentration in the reaction mixture. These factors influence the binding of primers to the target DNA sequence, thereby affecting the specificity of the amplification.

The Specificity or Stringency of a PCR Reaction Can Be