What technique can be used to determine the mass of a protein without knowing the identity of the molecule?
A) affinity chromatography
B) ion exchange chromatography
C) polyacrylamide gel electrophoresis
D) MALDI-TOF mass spectrometry
E) western blot

Question

Quizplus · Accepted Answer

MALDI-TOF mass spectrometry is a technique used to determine the mass of proteins, peptides, and other macromolecules without prior knowledge of their identity. It ionizes the sample with a laser and measures the time it takes for the ions to reach a detector, which is used to calculate their mass.

What Technique Can Be Used to Determine the Mass of a Protein