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What Is the Purpose of Synthesizing a Negative Strand RNA

Question 21

Multiple Choice

What is the purpose of synthesizing a negative strand RNA in positive stranded ssRNA viruses?


A) enable rolling circle amplification of the genome, which requires both strands of RNA
B) enable transcription of genes occurring on both the negative and positive strands of the genome, such as overlapping genes
C) proofreading of the genome to minimize mutations generated by the polymerase being passed onto virion progeny
D) to serve as the complementary template sequence in genome amplification of the positive strand

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