Inserting a kanamycin resistance cassette into a catabolic operon to confirm the gene is essential in degradation of a particular compound would involve all of the following EXCEPT
A) a reporter gene.
B) ligation.
C) recombination.
D) transformation.

Question

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Inserting a kanamycin resistance cassette into a catabolic operon to confirm the gene is essential in degradation of a particular compound involves ligation to attach the kanamycin resistance cassette to the operon, recombination to integrate the cassette into the organism's genome, and transformation to introduce the modified genome back into the organism. A reporter gene may not be necessary for this process as it is used to monitor gene expression and activity, but it could be useful for additional analysis.

Inserting a Kanamycin Resistance Cassette into a Catabolic Operon to Confirm