Hybridization requires a nucleic acid probe that binds to DNA or RNA of interest, which must be in the ________ form for hybridization to occur.
A) single-stranded
B) double-stranded
C) oligomerized
D) polymerized

Question

Quizplus · Accepted Answer

Hybridization requires the target nucleic acid to be in a single-stranded form to allow for complementary base pairing with the probe. Double-stranded DNA or RNA must be denatured or melted into single-stranded form before hybridization can occur. Oligomerized or polymerized forms of nucleic acids would not be suitable for hybridization as they would not allow for the necessary base pairing between the probe and target.

Hybridization Requires a Nucleic Acid Probe That Binds to DNA