
If you were carrying out a penicillin enrichment culture and you forgot to add penicillinase before plating the sample onto nutrient agar, what would happen?
A) You would get the same results whether you add this enzyme of not because penicillin naturally rapidly degrades in agar.
B) The prototrophs would be able to grow on the agar plates but the auxotrophs would not.
C) The auxotrophs would be able to grow on the agar plates but the prototrophs would not.
D) Prototrophs and auxotrophs would both be killed by the penicillin; only Penᴿ mutants would grow and you would not enrich for auxotrophs.
E) Prototroph and auxotroph colonies would change color from cream to red in the presence of the penicillin.
Correct Answer:
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