Double-stranded DNA will separate into two strands when exposed to
A) high temperature AND low pH
B) high pH AND low salt
C) low temperature AND high salt
D) low salt AND low pH
E) high temperature AND high pH.

Question

Quizplus · Accepted Answer

Double-stranded DNA is stabilized by hydrogen bonds between complementary base pairs. Exposure to high temperature may break these bonds, leading to the separation of the two strands. High pH, on the other hand, may cause the ionization of nucleotides and disrupt the hydrogen bonding within the DNA, but it may not be sufficient to cause strand separation. High temperature and high pH may lead to the most effective and rapid separation of DNA strands.

Double-Stranded DNA Will Separate into Two Strands When Exposed to