You are examining the DNA profile of an unknown pathogen. The pathogen's DNA has been digested with EcoRI restriction enzyme. There are 7 bands on DNA on the gel. Which of the following is NOT True?
A) The original piece of DNA has seven EcoRI restriction sites.
B) The original piece of DNA has six EcoRI restriction sites.
C) The DNA has not been completely restricted.
D) The restriction enzyme has been denatured.
E) The restriction enzyme EcoRI was isolated from E. coli.

Question

You are examining the DNA profile of an unknown pathogen. The pathogen's DNA has been digested with EcoRI restriction enzyme. There are 7 bands on DNA on the gel. Which of the following is NOT True?
A) The original piece of DNA has seven EcoRI restriction sites.
B) The original piece of DNA has six EcoRI restriction sites.
C) The DNA has not been completely restricted.
D) The restriction enzyme has been denatured.
E) The restriction enzyme EcoRI was isolated from E. coli.

Quizplus · Accepted Answer

The presence of 7 bands indicates that the DNA has been cut into 7 fragments, which means there are 6 EcoRI restriction sites, not 7.

You Are Examining the DNA Profile of an Unknown Pathogen