You are sent three clinical samples and are asked to determine whether they are the same strain. You perform standard identification techniques but cannot come to a conclusion. You decide to do an RFLP test. You digest the genomes of the organisms with the same restriction enzyme and run a gel. Each organism yields 5 bands on the gel, of the following sizes:
Organism A: 3 kb, 7 kb, 11 kb; 14 kb, 21 kb
Organism B: 3 kb, 7 kb, 2 kb, 8 kb, 11 kb
Organism C: 11 kb, 3 kb, 14 kb, 7 kb; 21 kb
What conclusion(s) can you make from this result?
A) Organisms A and B are likely the same strain but are different from organism C.
B) Organisms B and C are likely the same strain but are different from organism A.
C) Organisms A and C are likely the same strain but are different from organism B.
D) The organisms are all likely the same strain.
E) The organisms are all likely different strains.

Question

You are sent three clinical samples and are asked to determine whether they are the same strain. You perform standard identification techniques but cannot come to a conclusion. You decide to do an RFLP test. You digest the genomes of the organisms with the same restriction enzyme and run a gel. Each organism yields 5 bands on the gel, of the following sizes: 
Organism A: 3 kb, 7 kb, 11 kb; 14 kb, 21 kb
Organism B: 3 kb, 7 kb, 2 kb, 8 kb, 11 kb
Organism C: 11 kb, 3 kb, 14 kb, 7 kb; 21 kb
What conclusion(s) can you make from this result?
A) Organisms A and B are likely the same strain but are different from organism C.
B) Organisms B and C are likely the same strain but are different from organism A.
C) Organisms A and C are likely the same strain but are different from organism B.
D) The organisms are all likely the same strain.
E) The organisms are all likely different strains.

Quizplus · Accepted Answer

Organisms A and C have the same band sizes but in a different order, suggesting they are the same strain but have undergone genetic rearrangement. Organism B has a unique 2 kb band, indicating it is a different strain. Therefore, A and C are likely the same strain but different from B. There is not enough evidence to suggest that all three organisms are the same strain or that they are all different strains.

You Are Sent Three Clinical Samples and Are Asked to Determine