Which of the following is NOT a method used to differentiate E. coli O157:H7 from other strains based directly on genotype?
A) Fluorescence in situ hybridization (FISH)
B) Nucleic acid amplification tests (NAATs)
C) Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)
D) 16S rRNA gene sequencing
E) Radioisotope-labeled probes or fluorescent dye-labeled probes

Question

Which of the following is NOT a method used to differentiate E. coli O157:H7 from other strains based directly on genotype?
A) Fluorescence in situ hybridization (FISH)
B) Nucleic acid amplification tests (NAATs)
C) Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)
D) 16S rRNA gene sequencing
E) Radioisotope-labeled probes or fluorescent dye-labeled probes

Quizplus · Accepted Answer

Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is not a method used to differentiate E. coli O157:H7 from other strains based directly on genotype. FISH, NAATs, 16S rRNA gene sequencing, and radioisotope-labeled probes or fluorescent dye-labeled probes are all used to identify specific genetic markers that differentiate E. coli O157:H7 from other strains. MALDI-TOF MS is a method of mass spectrometry used to identify microbial organisms based on their protein profiles, not their genotype.

Which of the Following Is NOT a Method Used to Differentiate