A 1:20 dilution of blood is made for a white count,and the following number of cells counted in the four large corner mm² of the hemocytometer: 12,28,18,and 15.What should be done next?

Question

Quizplus · Accepted Answer

The distribution of cells in these four mm² is unacceptable (exceeds 10 cells);this is not random distribution.Unacceptable distribution makes the cell count invalid.Ideally,another dilution should be made,and it should be well mixed before charging the chamber.Other possible reasons for poor distribution include uneven flow when charging the chamber,dirt or oil on the surface of the chamber or coverslip,and disturbing the coverslip while counting the cells.

A 1:20 Dilution of Blood Is Made for a White