Which of the following will cause spuriously prolonged clot-based coagulation tests?

Question

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Filling an evacuated 3.2% sodium citrate only halfway (50%)will lead to falsely prolonged clot-based coagulation test results.The relative excess anticoagulant will bind (some)of the calcium added in the test system.A 90% fill gives valid results and can be used.Prolonged tourniquet application and plasma collected from a traumatic tap both have the potential of activating coagulation,thus leading to shortened,not prolonged,results.

Which of the Following Will Cause Spuriously Prolonged Clot-Based Coagulation