Which of the following practices is necessary when collecting blood specimen from a heparin or saline lock?

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Collection of blood from a heparin or saline lock is usually performed by physicians or nursing staff.The IV line must be flushed with 5-mL saline before the blood draw to avoid specimen contamination.The first 5 mL of blood that is collected should be discarded.IV lines should never be flushed with heparin before collecting blood for coagulation testing.Blood should be collected into a syringe,and a transfer device is used to transfer the blood to an evacuated tube.

Which of the Following Practices Is Necessary When Collecting Blood