Of these steps,which one occurs earliest in the process of producing recombinant DNA?
A)Human DNA fragments are mixed with the cut plasmids.
B)The same restriction enzyme is used to isolate the gene of interest and to cut the plasmid DNA.
C)The recombinant plasmids are mixed with bacteria.
D)Bacteria carrying recombinant plasmids are cloned.

Question

Quizplus · Accepted Answer

The use of a restriction enzyme to isolate the gene of interest and to cut the plasmid DNA occurs earliest in the process of producing recombinant DNA. This step allows for the creation of complementary sticky ends on both the gene of interest and the plasmid, facilitating their connection through ligation. The other steps listed occur after the gene has been inserted into the plasmid DNA.

Of These Steps,which One Occurs Earliest in the Process of Producing