During a sequencing reaction, the researcher adds all the required components to the reaction, but accidentally adds only one of the four dideoxynucleotides, the ddC. What kinds of products will be observed after this reaction is complete?

Question

Quizplus · Accepted Answer

In a sequencing reaction, when only one type of dideoxynucleotide (ddNTP) is added, in this case, ddC, all the resulting DNA fragments will terminate at the positions where a cytosine (C) would normally be incorporated. This is because ddNTPs lack the 3' hydroxyl group necessary for forming a phosphodiester bond with the next nucleotide, thus terminating DNA synthesis at that point. Therefore, all fragments will end in C.

During a Sequencing Reaction, the Researcher Adds All the Required