Which order of the following steps provides the most logical sequence to follow when splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non-plasmid DNA.
A) II, III, V, IV, I
B) III, II, IV, V, I
C) III, IV, V, I, II
D) IV, V, I, II, III

Question

Quizplus · Accepted Answer

The correct sequence for splicing foreign DNA into a plasmid and inserting it into a bacterium starts with extracting plasmid DNA from bacterial cells (III), then cutting the plasmid DNA with restriction enzymes (II), followed by hydrogen-bonding the plasmid DNA to non-plasmid DNA fragments (IV), using ligase to seal the plasmid DNA to non-plasmid DNA (V), and finally transforming bacteria with the recombinant DNA molecule (I).

Which Order of the Following Steps Provides the Most Logical