Which of the following characteristics allows the CRISPR Cas9 enzyme to be an effective way to generate mutations that block the function of a specific protein (knock out)?
A) It makes double stranded breaks in DNA throughout the genome.
B) It's function is regulated by a complementary guide RNA.
C) It forms a complex with DNA.
D) It removes random DNA bases.

Question

Quizplus · Accepted Answer

CRISPR Cas9's effectiveness in generating specific mutations is largely due to its use of a complementary guide RNA (gRNA) that directs the Cas9 enzyme to a specific location in the genome where it can make a double-stranded break. This allows for targeted editing of genes.

Which of the Following Characteristics Allows the CRISPR Cas9 Enzyme