Exposing Inner Mitochondrial Membranes to Ultrasonic Vibrations Will Fragment the Membranes

Exposing inner mitochondrial membranes to ultrasonic vibrations will fragment the membranes and pieces will reseal to form small vesicles that contain the intermembrane space. These vesicles can transfer electrons from NADH to oxygen and synthesize ATP. Which of the following statements best describes what will happen to the vesicles when NADH is added?

A) The inside of the vesicles will become acidic.
B) The inside of the vesicles will become alkaline.
C) ATP will be produced from ADP and i in the interior of the vesicle.
D) Protons will be pumped out of the interior of the vesicle to the exterior using energy from ATP hydrolysis.