Michelle wants to know which cells in a particular organ produce a specific gene product. She has a cDNA clone of the gene available. What method would be the most useful to answer this question?
A) Sanger sequencing of the clone
B) real time PCR
C) FISH (fluorescent in situ hybridization)
D) restriction fragment analysis
E) microinjection of the gene fragment

Question

Quizplus · Accepted Answer

FISH (fluorescent in situ hybridization) can be used to visualize the location of a specific gene in cells. By hybridizing the cDNA clone to the cells, Michelle can determine which cells express the gene product and therefore determine which cells produce it. Sanger sequencing, real time PCR, restriction fragment analysis, and microinjection are not appropriate methods for answering this question.

Michelle Wants to Know Which Cells in a Particular Organ