The technique known as real-time PCR is used to
A) prepare cDNA molecules for cloning.
B) detect the presence of specific genetic sequences in a sample.
C) isolate DNA fragments for cloning.
D) measure the number of copies of a DNA sequence in a sample.
E) both detect and isolate DNA fragments in a sample.

Question

Quizplus · Accepted Answer

Real-time PCR (Polymerase Chain Reaction) is a technique used to measure the number of copies of a specific DNA sequence in a sample. It is based on the amplification of the DNA target sequence and the detection of the amplified product in real-time with the use of fluorescent probes. This technique is commonly used in research and diagnostics to identify and quantify pathogens, monitor gene expression, and detect genetic mutations. It is not used for preparing cDNA molecules, isolating DNA fragments, or both detecting and isolating DNA fragments in a sample.

The Technique Known as Real-Time PCR Is Used to