The substrate for the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase is glyceraldehyde 3-phosphate (with one phosphate group) while its product is 1,3-bisphosphoglycerate (with two phosphate groups). Where does the extra phosphate group come from?

Question

Quizplus · Accepted Answer

In step 6 of glycolysis, this enzyme couples the oxidation of the substrate with the production of NADH, as well as incorporation of inorganic phosphate. The Pᵢ is then transferred to ADP to generate ATP in step 7.

The Substrate for the Glycolytic Enzyme Glyceraldehyde 3-Phosphate Dehydrogenase Is