This protein is present at every replication fork and prevents DNA polymerase from dissociating, but does not impede the rapid movement of the enzyme. Which of the following is true regarding this protein?
A) It self-assembles onto DNA at the replication fork.
B) It is assembled on DNA as soon as DNA polymerase runs into a double-strand region of DNA.
C) Its assembly normally follows the synthesis of a new primer by the DNA primase.
D) It disassembles from DNA as soon as DNA polymerase runs into a double-strand region.
E) All of the above.

Question

Quizplus · Accepted Answer

The sliding clamp is normally loaded (more frequently on the lagging strand) on the primer-template duplex by the clamp loader, a process that requires ATP hydrolysis by the loader. As soon as the polymerase runs into a double-strand region downstream, the clamp releases the polymerase but is not itself immediately disassembled from DNA.

This Protein Is Present at Every Replication Fork and Prevents