Indicate true (T) and false (F) statements below regarding the accuracy of translation by the ribosome. Your answer would be a four-letter string composed of letters T and F only, e.g. TFFF.
( ) The difference in the binding affinity of a codon to a correct versus incorrect anticodon CANNOT by itself account for the high accuracy of translation.
( ) GTP hydrolysis by EF-Tu both speeds up translation and increases its accuracy.
( ) The ribosome can detect correct Watson-Crick base-pairing between the codon and anticodon in the A site, and consequently trigger GTP hydrolysis by EF-Tu.
( ) Even after GTP hydrolysis by EF-Tu, the ribosome can prevent wrong amino acid incorporation by kinetic proofreading.

Question

Indicate true (T) and false (F) statements below regarding the accuracy of translation by the ribosome. Your answer would be a four-letter string composed of letters T and F only, e.g. TFFF.
( )	The difference in the binding affinity of a codon to a correct versus incorrect anticodon CANNOT by itself account for the high accuracy of translation.
( )	GTP hydrolysis by EF-Tu both speeds up translation and increases its accuracy.
( )	The ribosome can detect correct Watson-Crick base-pairing between the codon and anticodon in the A site, and consequently trigger GTP hydrolysis by EF-Tu.
( )	Even after GTP hydrolysis by EF-Tu, the ribosome can prevent wrong amino acid incorporation by kinetic proofreading.

Quizplus · Accepted Answer

The difference in affinity between correct and incorrect codon-anticodon matches cannot by itself account for the high accuracy of translation. The ribosome further assesses the correctness of the match; in the case of a correct match, EF-Tu hydrolyzes its bound GTP. This contributes to the speed and fidelity of translation. Even after EF-Tu release, there is a second proofreading opportunity for the ribosome to prevent an incorrect amino acid from being added to the growing chain.

Indicate True (T) and False (F) Statements Below Regarding the Accuracy