In isoelectric focusing, is the low-pH end close to the anode (A; positive electrode) or cathode (C)? Would you use octylglucoside (O; a nonionic detergent) or SDS (S) for separation of proteins by this technique? Would a highly basic protein be near the high-pH (H) or low-pH (L) end of the strip at the end of the experiment? Write down your answer as a three-letter string using the letters in the parentheses above, e.g. ASH.

Question

Quizplus · Accepted Answer

If the pH is lower than the isoelectric point of a protein, the protein is protonated (positively charged) and should therefore be repulsed by the nearby anode toward regions with higher pH. Ionic detergents are not suitable for this charge-sensitive technique. A basic protein has a high (basic) isoelectric point.

In Isoelectric Focusing, Is the Low-PH End Close to the Anode