Indicate whether ordinary agarose-gel electrophoresis (A), polyacrylamide-gel electrophoresis (P), or pulsed-field gel electrophoresis (F) is used to better separate each of the following DNA molecules. Your answer would be a three-letter string composed of letters A, P, and F only, e.g. APF.
( ) Small DNA oligonucleotides such as hybridization probes and PCR primers
( ) Plasmid DNA
( ) Bacterial chromosomes

Question

Indicate whether ordinary agarose-gel electrophoresis (A), polyacrylamide-gel electrophoresis (P), or pulsed-field gel electrophoresis (F) is used to better separate each of the following DNA molecules. Your answer would be a three-letter string composed of letters A, P, and F only, e.g. APF.
( )	Small DNA oligonucleotides such as hybridization probes and PCR primers
( )	Plasmid DNA
( )	Bacterial chromosomes

Quizplus · Accepted Answer

Whereas plasmid DNA of different sizes can be readily separated by ordinary agarose-gel electrophoresis, bacterial chromosomes can be best separated by a variation of this technique called pulsed-field gel electrophoresis. On the other hand, much smaller DNA molecules are best resolved by polyacrylamide-gel electrophoresis.

Indicate Whether Ordinary Agarose-Gel Electrophoresis (A), Polyacrylamide-Gel Electrophoresis (P), or Pulsed-Field