When the gene encoding a certain cytoskeleton protein is deleted, the resulting mutant cells round up and do not form their normal appendages. These mutants can be rescued when a gene encoding an N-terminal green fluorescent protein (GFP) fusion of the protein is expressed, but not when a gene encoding a C-terminal GFP fusion is expressed. Which fusion protein (N or C) is appropriate to use in studying cellular localization and activity? Write down N or C as your answer.

Question

Quizplus · Accepted Answer

If the gene encoding the fusion cannot rescue a loss-of-function mutation of the original gene, the presence of the fused protein might be interfering with gene function.

When the Gene Encoding a Certain Cytoskeleton Protein Is Deleted