You have isolated mitochondria from a liver tissue sample, suspended them in a hypotonic buffer that causes them to swell and burst their outer membrane, and then added sucrose to a final concentration of about 25%. You then layer the suspension on a density gradient, ultracentrifuge, collect the different fractions, and analyze their protein content. Which of the following proteins would you expect to be highly enriched in the lower density and higher density fractions, respectively?

Question

Quizplus · Accepted Answer

The low-density fractions would mostly contain outer-membrane components such as porins, while the high-density fractions correspond to the matrix and inner membrane.

You Have Isolated Mitochondria from a Liver Tissue Sample, Suspended