Which of the Following Best Explains the Fact That Patient

Which of the following best explains the fact that patient specimens are typically assayed at saturating substrate concentrations so that v is at Vmₐₓ?

A) To ensure that only the concentration of enzyme in the specimen affects the measured rate
B) To fit the data onto the standard curve generated by "y = mx + b"
C) To more closely mimic the enzymatic reaction as it occurs in vivo where enzymes generally only function at Vmₐₓ
D) To prevent inactive enzyme molecules from being mistakenly counted as substrate.