Which substance would you expect to be eluted first from a molecular sieve column with a suitable degree of crosslinking?
A) hemoglobin
B) myoglobin
C) 2,3- bis phosphoglycerate
D) all would elute at the same rate

Question

Quizplus · Accepted Answer

In a molecular sieve column, larger molecules elute first because they cannot enter the pores of the stationary phase and thus travel through the column faster. Hemoglobin, being the largest molecule among the options, would elute first.

Which Substance Would You Expect to Be Eluted First from a Molecular